演者： Dr. Takayasu Mikuni (三國 貴康先生) Brain Research Institute, Niigata University（新潟大学）
演題: "Genome Editing in Mammalian Brain in vivo: Key Technologies and Future Applications"
要旨:Rapid and precise genome editing using CRISPR-Cas systems is a powerful technology in various scientific fields. Especially, genome editing through homology-directed repair (HDR) provides a versatile approach to precisely introduce insertions, deletions or replacements in the genome. However, it has been considered to be difficult to induce HDR in postmitotic neurons, limiting the application of precise genome editing in the field of neuroscience. Here Dr. Mikuni presents two strategies, SLENDR and vSLENDR, to induce HDR- mediated genome editing in mammalian brain in vivo (Cell 2016, Neuron 2017). Rapid and precise genome editing in the brain will allow for a wide range of applications in neuroscience research, such as high-throughput monitoring and manipulation of endogenous gene products in brain tissue.
